磷酸二酯酶I
中文名稱： 磷酸二酯酶I
其他中文名稱： 磷酸二脂酶；磷酸二酯酶
英文名稱： Phosphodiesterase I from Crotalus adamanteus venom
其他英文名稱： 5′-Exonuclease；Oligonucleate 5′-nucleotidohydrolase
產(chǎn)品規(guī)格： BR，20u/mg
包裝： 100U
CAS號(hào)：9025-82-5
磷酸二酯酶I級(jí)別：BR
Activity：≥20u/mg
活力定義：One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25℃, pH 8.9.
產(chǎn)品描述：Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+
性狀：白色或類白色粉末
用途：生化研究。
磷酸二酯酶I保存：-20℃